• Users Online: 793
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2015  |  Volume : 18  |  Issue : 2  |  Page : 95-99

Early dengue diagnosis: Role of rapid NS1 antigen, NS1 early ELISA, and PCR assay


Department of Microbiology, Seth Gordhandas Sunderdas Medical College and King Edward Memorial Hospital, Mumbai, Maharashtra, India

Correspondence Address:
Dr. Vaishali N Solanke
Department of Microbiology, 5th Floor, MSB Seth Gordhandas Sunderdas Medical College and King Edward Memorial Hospital, Parel, Mumbai - 400 012, Maharashtra
India
Login to access the Email id


DOI: 10.4103/1119-0388.158402

Rights and Permissions

Context: Early and accurate dengue diagnosis is important for disease surveillance, and also to start effective control measures in endemic countries. Aims: In this study, we evaluated the performance of three techniques available for early dengue diagnosis, viz., rapid nonstructural 1 (NS1) antigen test, NS1 early enzyme-linked immunosorbent assay (ELISA) test, and reverse transcription polymerase chain reaction (RT-PCR) test. Settings and Design: Retrospective analytical study. Materials and Methods: Performance of three different techniques, viz., rapid NS1 antigen, NS1 early ELISA, and RT-PCR on serum samples of suspected dengue cases. Statistical Analysis Used: Sensitivity, specificity, negative predictive value, and positive predictive value calculated for rapid test and ELISA test, keeping PCR as the gold standard Results: Amongst 155 samples received from clinically-suspected dengue cases within 1-9 days of fever, 56 samples were received during 1-3 days, 52 samples were received during 4-6 days, and 47 samples were received during 7-9 days. Of these 155 samples, 38 (24.5%) were positive by rapid NS1 antigen, 47 (30.3%) by NS1 ELISA, and 54 (34.8%) by reverse transcriptase polymerase chain reaction (RT-PCR). Rapid NS1 antigen and NS1 ELISA showed the highest positivity on days 1-3, while highest positivity for PCR was on days 4-6. The sensitivity and specificity of NS1 ELISA were 66.6% and 89.1%, while sensitivity and specificity of rapid NS1 antigen were 55.5% and 92%, respectively. Conclusions: With high mortality and morbidity associated with dengue, it is imperative to diagnose the disease during the early phase. All three assay formats are helpful in early diagnosis and to provide information of dengue cases in time.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed10822    
    Printed75    
    Emailed0    
    PDF Downloaded683    
    Comments [Add]    
    Cited by others 5    

Recommend this journal