• Users Online: 162
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2016  |  Volume : 19  |  Issue : 2  |  Page : 94-99

Bacteriological profile and antibiogram of blood culture isolates from a tertiary care hospital of North India


Department of Microbiology, Lok Nayak Hospital, Maulana Azad Medical College, New Delhi, India

Correspondence Address:
Bineeta Kashyap
Department of Microbiology, Lok Nayak Hospital, Maulana Azad Medical College, New Delhi
India
Login to access the Email id


DOI: 10.4103/1119-0388.185426

Rights and Permissions

Introduction: Blood stream infections cause a significant morbidity and mortality worldwide. Rapid and reliable detection of bacterial pathogens and rational use of antimicrobials are required for proper management. The present work was undertaken to study the bacteriological profile along with the antibiogram of the blood culture isolates from clinically diagnosed cases of sepsis. Materials and Methods: During the 1-year study period, 3472 blood samples from patients with a clinical diagnosis of sepsis were received at the Emergency Microbiology Laboratory of a 2500-bedded tertiary care hospital of North India. Bacteriological identification and antimicrobial susceptibility testing were performed for all bacterial isolates by following the standard protocol. Results: Culture positivity was seen in 16.5% of the septicemic cases. The most common bacteria isolated were Escherichia coli (22.4%) followed by Klebsiella species (19.7%), Staphylococcus aureus (18.3%), and coagulase-negative staphylococci (17.4%). About 26.5% of S. aureus were methicillin-resistant. Vancomycin (100%), gentamicin (87.9%), and ciprofloxacin (73%) showed the highest activity among the Gram-positive isolates. Most of the Gram-negative bacteria were multi-drug resistant (67.1%). Imipenem (98.8%), amikacin (90.8%), and cefoperazone/sulbactam combination (81.1%) showed the highest activity among Enterobacteriaceae. Nonfermenters in majority were susceptible to imipenem (88.2%), amikacin (81.6%), and piperacillin/tazobactam combination (72.4%). Gram-negative isolates showed 100% sensitivity toward colistin. Conclusion: The present study highlights the bacteriological etiology of sepsis along with the antibiogram of septicemic isolates that may provide necessary information for the formulation of antibiotic policy in effective management of such cases.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2677    
    Printed66    
    Emailed0    
    PDF Downloaded494    
    Comments [Add]    
    Cited by others 1    

Recommend this journal