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ORIGINAL ARTICLE
Year : 2017  |  Volume : 20  |  Issue : 2  |  Page : 196-200

Comparison of conventional and rapid methods for the detection of methicillin resistance in Staphylococcus aureus from blood cultures


Department of Microbiology, University College of Medical Sciences, Guru Teg Bahadur Hospital, New Delhi, India

Correspondence Address:
Ashwani Kumar
Department of Microbiology, University College of Medical Sciences, Guru Teg Bahadur Hospital, New Delhi - 110 095
India
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DOI: 10.4103/tjmr.tjmr_48_16

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Background: Considering the increasing rate of infections caused by methicillin-resistant Staphylococcus aureus (MRSA), reliable, accurate, and rapid testing for the detection of MRSA is essential for both initiation of antimicrobial therapy and infection control measures. Materials and Methods: Fifty clinical isolates of S. aureus from patients with bacteremia were confirmed bacteriologically and subjected to antimicrobial susceptibility testing, followed by the detection of methicillin resistance by oxacillin and cefoxitin disc diffusion methods, oxacillin agar screen test, and direct cefoxitin disc diffusion method from blood culture bottles. Results: On comparing the methods under study, 36% MRSA were detected by disc diffusion methods, whereas 44% and 46% were detected by oxacillin agar screen and direct cefoxitin disc diffusion blood culture, respectively. Conclusions: The emergence of MRSA underscores the need for programs to prevent the spread of antimicrobial-resistant organisms and control the use of antimicrobial drugs in health-care settings.


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